LXA4 (Lipoxin A4) ELISA Kit - ELK7851

LXA4 (Lipoxin A4) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7851-48, ELK7851-96, ELK7851-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: LX-A4

Assay Type: Competitive Inhibition

Sensitivity: 172.4 pg/mL

Standard: 40000 pg/mL

Detection Range: 625-40000 pg/mL

Sample Type: serum, plasma, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Signal transduction; Metabolic pathway; Infection immunity;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LXA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LXA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LXA4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only