Magnetic Beads-conjugated Rabbit Anti GFP-Tag mAb
Size: 100μg
Catalogue Number: AE118-100
Citations, Manuals and MSDS Available upon request.
Background: The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.
Category: Tag Antibodies
Applications: IP
Cross-reactivity: Species independent
Observed Molecular Weight: Refer to figures
Immunogen: Recombinant protein (or fragment).
Alternate Names: GFP;GFP tag;GFP-tag
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, pH 7.3.
Recommended Dilutions: IP, 20μl-40μl Magnetic Beads for 100μg-300μg extracts of whole cells
NCBI Alias: GFP
Clone Number Info: ARC50813
Research Use Only