Product Description

MAK (phospho Tyr159) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6167-50, ES6167-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: MAK, Serine/threonine-protein kinase MAK, Male germ cell-associated kinase

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human MAK around the phosphorylation site of Tyr159. AA range:126-175

Isotype: IgG

Target: MAK

Specificity: Phospho-MAK (Y159) Polyclonal Antibody detects endogenous levels of MAK protein only when phosphorylated at Y159.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines, Isoform 1 is expressed in kidney, testis, lung, trachea, and retina, Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors,

Observed Band (KD): 85kD

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Function: Could play an important function in spermatogenesis
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily
Similarity: Contains 1 protein kinase domain
Tissue Specificity: Expressed mainly in testicular cells at and after meiosis

Subcellular Location: Nucleus Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Midbody Cell projection, cilium, photoreceptor outer segment Photoreceptor inner segment Localized in both the connecting cilia and the outer segment axonemes (By similarity) Localized uniformly in nuclei during interphase, to the mitotic spindle and centrosomes during metaphase and anaphase, and also to midbody at anaphase until telophase

Storage and Stability: -20°C/1 year

Gene Name: MAK

Protein Name: Serine/threonine-protein kinase MAK

Mouse Gene ID: 17152

Mouse SwissProt Number: Q04859

Rat Gene ID: 25677

Rat SwissProt Number: P20793

Research Use Only