Product Description

MARK2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2747-50, ES2747-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: microtubule affinity regulating kinase 2(MARK2) Homo sapiens This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009],

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Applications: WB, IF, ELISA

Alternate Names: MARK2, EMK1, Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, EMK-1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, Par-1b, Par1b

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human MARK2. AA range:10-59

Isotype: IgG

Target: MARK2

Specificity: MARK2 Polyclonal Antibody detects endogenous levels of MARK2 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney,

Observed Band (KD): 85kD

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Cofactor: Magnesium
Enzyme Regulation: Activated by phosphorylation on Thr-208 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39
Function: Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily
Similarity: Contains 1 KA1 (kinase-associated) domain
Similarity: Contains 1 protein kinase domain
Similarity: Contains 1 UBA doma

Subcellular Location: Cell membrane, Peripheral membrane protein Cytoplasm Lateral cell membrane Cytoplasm, cytoskeleton Cell projection, dendrite Cytoplasm Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: MARK2

Protein Name: Serine/threonine-protein kinase MARK2

Mouse Gene ID: 13728

Mouse SwissProt Number: Q05512

Rat Gene ID: 60328

Rat SwissProt Number: O08679

Research Use Only