MEK (Methionine-Enkephalin) ELISA Kit - ELK9576

MEK (Methionine-Enkephalin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9576-48, ELK9576-96, ELK9576-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Met-enkephalin; Opioid growth factor; OGF

Assay Type: Competitive Inhibition

Sensitivity: 77 pg/mL

Standard: 8000 pg/mL

Detection Range: 125.00-8000 pg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Signal Transduction

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MEK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MEK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MEK in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only