Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit - E-BC-K149-M

Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K149-M-48, E-BC-K149-M-96

Citations, Manuals and MSDS Available upon request.

Research Areas: Mitochondria Function;Cuproptosis

Result Type: Enzyme Activity

Detection Instrument: Microplate reader

Tested Sample Types: animal tissue

Assay Time: 15 min

Detection Type: Colorimetric method

Sensitivity: 15.58 U/L

Detection Range: 15.58-224.2 U/L

Test Principle: Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The specific inhibitor rotenone can inhibit the enzyme, and the total enzyme activity minus the enzyme activity after inhibitor inhibition can reflect its specific enzyme activity.

Inter CV: 8.4

Intra CV: 0.8

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag