Mouse β-CTx (Beta Crosslaps) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-EL-M0372-24, E-EL-M0372-48, E-EL-M0372-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: β-CTx
UNIProt ID: P11087（COL1A1）
Target Synonyms: b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction;
Research Areas: Signal transduction, Metabolism
Target Species: Mouse
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse β-CTx concentrations in serum, plasma and other biological fluids.
Tested Sample Types: Serum, Plasma and other biological fluids
Type: Competitive
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivity: 46.88 pg/mL
Detection Range: 78.13-5000 pg/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-CTx. During the reaction, Mouse β-CTx in the sample or standard competes with a fixed amount of Mouse β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Mouse β-CTx in samples. No significant cross-reactivity or interference between Mouse β-CTx and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃/-20℃, 12 months
Shipping: ice bag