Mouse 20S-PSM (20S-Proteasome) ELISA Kit - ELK6913

Mouse 20S-PSM (20S-Proteasome) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6913-48, ELK6913-96, ELK6913-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CP; 20SPSM; 20S Core Particle

Assay Type: Competitive Inhibition

Sensitivity: 155.1 ng/mL

Standard: 30000 ng/mL

Detection Range: 468.75-30000 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Enzyme and Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 20S-PSM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only