Product Description

Mouse 4-HNE (4-Hydroxynonenal) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8374MS-48, ELK8374MS-96, ELK8374MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal

Reactivity: Mouse

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Metabolic Pathway, Infection Immunity, Hormone Metabolism, Dermatology

Assay Length: 3.5h

Sensitivity: 1.1 ng/mL

Standard: 150 ng/mL

Detection Range: 2.35-150 ng/mL

Research Use Only