Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit - ELK5551

Mouse a1M (Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5551-48, ELK5551-96, ELK5551-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin

Assay Type: Competitive Inhibition

Sensitivity: 0.97 µg/mL

Standard: 200 µg/mL

Detection Range: 3.13-200 µg/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Infection immunity; Immune molecule; Kidney biomarker;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse a1M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1M in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only