Mouse Acly (ATP-citRate synthase) ELISA Kit - ELK0597

Mouse Acly (ATP-citRate synthase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0597-48, ELK0597-96, ELK0597-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ACL; ACLY; ATP citrate (pro S ) lyase; ATP citrate lyase; ATP citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP

Assay Type: Sandwich

Sensitivity: 0.266 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabonomics

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Acly. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Acly. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Acly, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Acly in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q91V92

Research Use Only