Product Description

Mouse ACP3 (Acid Phosphatase 3, Prostatic) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11834-48, ELK11834-96, ELK11834-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Mouse

Alternate Names: Prostatic acid phosphatase,Acp3,5'-nucleotidase,Acid phosphatase 3,Fluoride-resistant acid phosphatase,Ecto-5'-nucleotidase,Thiamine monophosphatase,Acpp

Sensitivity: 35.3 pg/mL

Assay Type: Sandwich

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Enzyme And Kinase, Metabolic Pathway, Tumor Immunity, Bone Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q8CE08

Research Use Only