Mouse AGRP (Agouti Related Protein) ELISA Kit - ELK3764

Mouse AGRP (Agouti Related Protein) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK3764-48, ELK3764-96, ELK3764-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: AGRT; ASIP2; ART; Agouti-Related Peptide

Assay Type: Sandwich

Sensitivity: 12 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Metabolic pathway; Neuroscience; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AGRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AGRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AGRP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P56473

Research Use Only