Product Description

Mouse Alk-Smase (Alkaline Sphingomyelinase) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK2630-48, ELK2630-96, ELK2630-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: ENPP7, E-NPP 7, NPP-7, Ectonucleotide Pyrophosphatase/Phosphodiesterase 7, Sphingomyelin Phosphodiesterase, Intestinal alkaline sphingomyelinase

Reactivity: Mouse

Sample Type: Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Neuro Science

Assay Length: 3.5h

Sensitivity: 0.049 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: Q3TIW9

Research Use Only