Mouse Anti DDDDK-Tag mAb
Size: 100μg
Catalogue Number: AE005-100
Citations, Manuals and MSDS Available upon request.
Background: FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Category: Tag Antibodies
Applications: WB, IF/ICC, IP, FC (intra), ELISA
Cross-reactivity: Species independent
Observed Molecular Weight: 37kDa/48kDa/57kDa/70kDa
Immunogen: Synthetic peptide
Species: Human
Alternate Names: DDDDK;DDDDK tag;DDDDK-tag
Source: Mouse
Isotype: IgG1, Kappa
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:10000 - 1:80000 IF/ICC, 1:200 - 1:800 IP, 0.5μg-4μg antibody for 100μg-200μg extracts of whole cells FC (intra), 5 μl per 10^6 cells in 100 μl volume ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: DDDDK
Clone Number Info: AMC0382
Research Use Only