Mouse Anti His-Tag mAb
Size: 100μg
Catalogue Number: AE003-100
Citations, Manuals and MSDS Available upon request.
Background: Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
Category: Tag Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Species independent
Observed Molecular Weight: 35 kDa (Snail transfected) /58 kDa (CYP1A2 transfected)
Immunogen: Synthetic peptide
Alternate Names: His;His tag;His-tag
Source: Mouse
Isotype: IgG1
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:5000 - 1:30000 IF/ICC, 1:5000-1:10000 IP, 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: His
Clone Number Info: AMC0149
Research Use Only