Product Description

Mouse Anti-KLH IgM (Keyhole Limpet Hemocyanin IgM) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0110-48, ELK0110-96, ELK0110-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Mouse

Alternate Names: Keyhole limpet IgM

Sensitivity: 0.51 ng/mL

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates

Assay Length: 3.5h

Research Area: Immunology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-KLH IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-KLH IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-KLH IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-KLH IgM in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only