Mouse Anti V5-Tag mAb
Size: 100μg
Catalogue Number: AE017-100
Citations, Manuals and MSDS Available upon request.
Background: Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Category: Tag Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Species independent
Observed Molecular Weight: Refer to Figures
Immunogen: Synthetic peptide
Species: Human
Alternate Names: V5;V5 tag;V5-tag
Source: Mouse
Isotype: IgG2b, Kappa
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:2000 - 1:10000 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NCBI Alias: V5
Clone Number Info: AMC0506
Research Use Only