Mouse ATXN10 (Ataxin 10) ELISA Kit - ELK7277

Mouse ATXN10 (Ataxin 10) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7277-48, ELK7277-96, ELK7277-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: E46L; SCA10; Spinocerebellar Ataxia 10; Brain protein E46 homolog

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Neuroscience

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ATXN10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ATXN10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ATXN10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ATXN10 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P28658

Research Use Only