Mouse BCAT2 (Branched-chain-amino-acid aminotransferase, mitochondrial) ELISA Kit - ELK9527

Mouse BCAT2 (Branched-chain-amino-acid aminotransferase, mitochondrial) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9527-48, ELK9527-96, ELK9527-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT (m); Bcatm; Eca40; Bcat2

Assay Type: Sandwich

Sensitivity: 6.3 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BCAT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCAT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BCAT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BCAT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O35855

Research Use Only