Mouse BCHE (Butyrylcholinesterase) ELISA Kit - ELK7806

Mouse BCHE (Butyrylcholinesterase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7806-48, ELK7806-96, ELK7806-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: BuChE; CHE1; E1; Pseudocholinesterase; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II

Assay Type: Competitive Inhibition

Sensitivity: 0.88 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Enzyme and Kinase; Metabolic pathway; Neuroscience;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse BCHE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCHE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BCHE in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only