Product Description

Mouse Biotin ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10917-48, ELK10917-96, ELK10917-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Biotin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Biotin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Biotin in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Biotin

Reactivity: Mouse

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Enzyme and Kinase

Assay Length: 2h

Sensitivity: 4.7 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Research Use Only