Mouse C5a (Complement Component 5a) ELISA Kit - ELK1052

Mouse C5a (Complement Component 5a) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1052-48, ELK1052-96, ELK1052-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Complement Component 5a

Assay Type: Sandwich

Sensitivity: 0.57 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Infection immunity; Immune molecule; Autoimmunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C5a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C5a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C5a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C5a in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P06684

Research Use Only