Product Description

Mouse CA9 (Carbonic Anhydrase IX) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK10726-48, ELK10726-96, ELK10726-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CA9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CA-IX, CAIX, MN, pMW1, P54/58N, Carbonic Dehydratase, Renal cell carcinoma-associated antigen G250, RCC-Associated Protein G250, Carbonate dehydratase IX

Reactivity: Mouse

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase

Assay Length: 3.5h

Sensitivity: 10.1 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

UniProt: Q8VHB5

Research Use Only