Product Description

Mouse CAPN1 (Calpain 1, Large Subunit) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2714-48, ELK2714-96, ELK2714-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Mouse

Alternate Names: PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain

Sensitivity: 0.62 ng/mL

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Enzyme And Kinase, Neuro Science, Gastroenterology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CAPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CAPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CAPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CAPN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O35350

Research Use Only