Mouse CD83 (Cluster Of Differentiation 83) ELISA Kit - ELK3356

Mouse CD83 (Cluster Of Differentiation 83) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK3356-48, ELK3356-96, ELK3356-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: BL11; HB15; Activated B Lymphocytes,Immunoglobulin; B-cell activation protein; Cell surface protein HB15

Assay Type: Sandwich

Sensitivity: 18.57 pg/mL

Standard: 3000 pg/mL

Detection Range: 46.88-3000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: CD and Adhesion molecule; Infection immunity; Immune molecule; Dermatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD83. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD83. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD83, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD83 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O88324

Research Use Only