Mouse CD9 (CD9 antigen) ELISA Kit - ELK11315

Mouse CD9 (CD9 antigen) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11315-48, ELK11315-96, ELK11315-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CD9 antigen ; CD9

Assay Type: Sandwich

Sensitivity: 1.34 pg/mL

Standard: 300 pg/mL

Detection Range: 4.69-300 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Bone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD9 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P40240

Research Use Only