Mouse CP (Ceruloplasmin) ELISA Kit - ELK10421

Mouse CP (Ceruloplasmin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10421-48, ELK10421-96, ELK10421-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CP2; CER; Caeruloplasmin; Ferroxidase; Oxygen Oxidoreductase

Assay Type: Sandwich

Sensitivity: 0.59 µg/mL

Standard: 100 µg/mL

Detection Range: 1.57-100 µg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Metabolic pathway; Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q61147

Research Use Only