Mouse E-Cad (E-Cadherin) ELISA Kit - ELK11211

Mouse E-Cad (E-Cadherin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11211-48, ELK11211-96, ELK11211-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin

Assay Type: Sandwich

Sensitivity: 0.132 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal Transduction; Cancer; Developmental Biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P09803

Research Use Only