Product Description

Mouse EAAT1 (Excitatory Amino Acid Transporter 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7256-48, ELK7256-96, ELK7256-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EAAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EAAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EAAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse EAAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: SLC1A3, EA6, GLAST1, Sodium-dependent glutamate/aspartate transporter 1, Solute Carrier 1 Member 3, Glial High Affinity Glutamate Transporter, GLutamate Aspartate Transporter

Reactivity: Mouse

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Neuro Science

Assay Length: 3.5h

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: P56564

Research Use Only