Mouse ERK1/2 (Extracellular Signal Regulated Kinase 1/2) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0019-48, ELK0019-96, ELK0019-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: ERK; ERK-2; ERK2; ERT1; MAPK2; NS13; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
Standard: 50 ng/mL
Detection Range: 0.78-50 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Areas: Signal transduction; Enzyme and Kinase; Apoptosis; Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only