Mouse F11 (Coagulation Factor XI) ELISA Kit - ELK11319

Mouse F11 (Coagulation Factor XI) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11319-48, ELK11319-96, ELK11319-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Coagulation factor XI; FXI; Plasma thromboplastin antecedent (PTA); F11

Assay Type: Sandwich

Sensitivity: 0.19 ng/mL

Standard: 30 ng/mL

Detection Range: 0.47-30 ng/mL

Sample Type: plasma

Assay Length: 3.5h

Research Areas: Hematology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F11 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q91Y47

Research Use Only