Mouse F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit - ELK2760

Mouse F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2760-48, ELK2760-96, ELK2760-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain

Assay Type: Sandwich

Sensitivity: 1.45 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Plasma .

Assay Length: 3.5h

Research Areas: Signal transduction; Metabolic pathway; Hematology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F13B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F13B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F13B in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q8K2H3

Research Use Only