Mouse FABP3 (Fatty Acid Binding Protein 3, Muscle And Heart) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK4355MS-48, ELK4355MS-96, ELK4355MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Mouse
Alternative Names: SMPD2; N-SMase; ISC1; Neutral Sphingomyelin Phosphodiesterase; Sphingomyelin Phosphodiesterase 2, Neutral Membrane; Lyso-platelet-activating factor-phospholipase C
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: Enzyme and Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NSMASE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NSMASE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NSMASE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NSMASE in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O60906
Research Use Only