Mouse FAPa (Fibroblast Activation Protein Alpha) ELISA Kit - ELK6468

Mouse FAPa (Fibroblast Activation Protein Alpha) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6468-48, ELK6468-96, ELK6468-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: APCE; SIMP; DPPIV; Seprase; 170 kDa melanoma membrane-bound gelatinase; Post-proline cleaving enzyme; Serine integral membrane protease; Surface-expressed protease

Assay Type: Sandwich

Sensitivity: 13.1 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Tumor immunity; Dermatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FAPa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FAPa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FAPa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FAPa in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P97321

Research Use Only