Mouse FETUA (Fetuin A) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1232MS-48, ELK1232MS-96, ELK1232MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Mouse
Alternative Names: B61; ECKLG; EFL1; EPLG1; LERK1; TNFAIP4; EPH-related receptor tyrosine kinase ligand 1; Tumor necrosis factor alpha-induced protein 4; Immediate early response protein B61
Assay Type: Sandwich
Sensitivity: 22.5 pg/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNA1, and the Human EFNA1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EFNA1. After TMB substrate solution is added, only those wells that contain Human EFNA1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNA1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P20827
Research Use Only