Mouse FST (Follistatin) ELISA Kit - ELK10297

Mouse FST (Follistatin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10297-48, ELK10297-96, ELK10297-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: FST; Activin-binding protein; FS; FST; Follistatin

Assay Type: Sandwich

Sensitivity: 0.12 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Endocrinology; Reproductive science; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FST in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P47931

Research Use Only