Mouse FXR (Farnesoid X Receptor) Microsample ELISA Kit - ELK6339MS

Mouse FXR (Farnesoid X Receptor) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6339MS-48, ELK6339MS-96, ELK6339MS-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NR1H4; BAR; FXR; HRR1; RIP14; Nuclear Receptor Subfamily 1,Group H,Member 4; Bile acid receptor; Farnesol receptor HRR-1; Retinoid X receptor-interacting protein 14

Assay Type: Sandwich

Sensitivity: 0.049 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction; Metabolic pathway; Endocrinology; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FXR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FXR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FXR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FXR in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only