Mouse GADD45g (Growth Arrest And DNA Damage Inducible Protein Gamma) ELISA Kit - ELK8050

Mouse GADD45g (Growth Arrest And DNA Damage Inducible Protein Gamma) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8050-48, ELK8050-96, ELK8050-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CR6; DDIT2; GADD45γamma; GRP17; Gadd-Related Protein,17 kD; Cytokine-responsive protein CR6; DNA damage-inducible transcript 2 protein

Assay Type: Sandwich

Sensitivity: 0.224 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GADD45g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GADD45g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GADD45g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GADD45g in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9Z111

Research Use Only