Mouse GAGs (Glycosaminoglycan) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0069-48, ELK0069-96, ELK0069-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Mucopolysaccharide; GAGs
Assay Type: Competitive Inhibition
Sensitivity: 0.51 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Tumor immunity; Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GAGs. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAGs. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAGs in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only