Mouse GSH (Glutathione) ELISA Kit - ELK10913

Mouse GSH (Glutathione) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10913-48, ELK10913-96, ELK10913-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: L-Reduced Glutathione

Assay Type: Competitive Inhibition

Sensitivity: 0.34 µg/mL

Standard: 100 µg/mL

Detection Range: 1.56-100 µg/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse GSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GSH in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only