Mouse HDAC3 (Histone Deacetylase 3) ELISA Kit - ELK11427

Mouse HDAC3 (Histone Deacetylase 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11427-48, ELK11427-96, ELK11427-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HD3; Protein deacetylase HDAC3 ; Protein deacylase HDAC3

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HDAC3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HDAC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HDAC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HDAC3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O88895

Research Use Only