Product Description

Mouse HE4 (Epididymal protein 4) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0863-48, ELK0863-96, ELK0863-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HE4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HE4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HE4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Epididymis Protein 4, dJ461P17.6 , EDDM4 , epididymal protein 4 , Epididymal secretory protein E4 , Epididymis specific whey acidic protein type four disulfide core , HE 4 , Major epididymis specific protein E4 , Major epididymis-specific protein E4 , MGC57529 , Putative protease inhibitor WAP5 , WAP 5 , WAP domain containing protein HE4 , WAP domain containing protein HE4 V4 , WAP four disulfide core domain 2 , WAP four disulfide core domain protein 2 , WAP four-disulfide core domain protein 2 , WAP5 , WFDC 2 , WFDC2 , WFDC2_HUMAN

Reactivity: Mouse

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Ovarian Cancer

Assay Length: 3.5h

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: Q9DAU7

Research Use Only