Product Description

Mouse HSD11b1 (11-Beta-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7488-48, ELK7488-96, ELK7488-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSD11b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSD11b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSD11b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSD11b1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 11DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, SDR26C1, Corticosteroid 11-beta-dehydrogenase isozyme 1, Short Chain Dehydrogenase/Reductase Family 26C, Member 1

Reactivity: Mouse

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Metabolic Pathway, Endocrinology, Cardiovascular Biology, Neuro Science, Hepatology, Hormone Metabolism

Assay Length: 3.5h

Sensitivity: 0.11 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

UniProt: P50172

Research Use Only