Mouse I-PTH (IntactParathormone) Microsample ELISA Kit - ELK2564MS

Mouse I-PTH (IntactParathormone) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2564MS-48, ELK2564MS-96, ELK2564MS-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone

Assay Type: Sandwich

Sensitivity: 3.96 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway; Endocrinology; Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only