Product Description

Mouse IgG2a (Immunoglobulin G2a) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8143-48, ELK8143-96, ELK8143-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Mouse

Alternate Names: IgG2a

Sensitivity: 0.072 µg/mL

Assay Type: Sandwich

Standard: 10 µg/mL

Detection Range: 0.16-10 µg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Infection Immunity, Immune Molecule, Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IgG2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IgG2a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IgG2a in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only