Product Description

Mouse IL28B (Interleukin 28B) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11927-48, ELK11927-96, ELK11927-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Mouse

Alternate Names: IL-28B, IFNL3,Interferon lambda-3

Sensitivity: 3.1 pg/mL

Assay Type: Sandwich

Standard: 7.82 pg/mL

Detection Range: 500-7.82 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Cytokine, Signal Transduction, Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL28B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL28B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL28B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL28B in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q8CGK6

Research Use Only