Mouse KLK1 (Kallikrein 1) ELISA Kit - ELK10619

Mouse KLK1 (Kallikrein 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10619-48, ELK10619-96, ELK10619-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein

Assay Type: Competitive Inhibition

Sensitivity: 0.14 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Enzyme and Kinase; Tumor immunity; Hepatology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse KLK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P15947

Research Use Only