Mouse LAB7-2 (B-Lymphocyte Activation Antigen B7-2) ELISA Kit - ELK0306

Mouse LAB7-2 (B-Lymphocyte Activation Antigen B7-2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0306-48, ELK0306-96, ELK0306-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2,B7-2 Antigen; CTLA-4 counter-receptor B7.2

Assay Type: Sandwich

Sensitivity: 0.048 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; CD and Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only