Mouse Lp-a (Lipoprotein a) ELISA Kit - ELK11050

Mouse Lp-a (Lipoprotein a) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11050-48, ELK11050-96, ELK11050-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: LP; AK38; APOA

Assay Type: Sandwich

Sensitivity: 0.128 µg/mL

Standard: 20 µg/mL

Detection Range: 0.312-20 µg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Metabolic pathway; Cardiovascular biology; Hepatology; Gastroenterology; Nutrition metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lp-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lp-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lp-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Lp-a in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only